Oxford Expression Technologies offers a comprehensive custom baculovirus expression
service that can be tailored to individual requirements.
Now you can get the very best custom services from the world-wide specialists
in this field.
The information provided here is a general guide to our expertise.
We would be pleased to quote for additional services designed to suit your
requirements.
Cloning of gene into suitable baculovirus vector
- N- and C- terminal tag integration and protease cleavage site integration
optional.
- Range of vectors for multiple gene expression, containing signal
sequences for secreted targets and utilizing different promoters available
Production of recombinant virus
- flashBAC has the chitinase gene deleted, which improves yield of
most secreted and membrane targeted proteins.
- flashBACGOLD has the chitinase
and cathepsin genes deleted, which improves yield of most difficult to
express proteins.
- Fast production time.
- Can be automated for multiple simultaneous virus
production
Optimisation of expression
- Cell lines assessed for expression levels
- Optimal multiplicity of infection
determination may save on inoculum for subsequent protein production
- Optimal
time point for harvest may save time/increase yield
Protein purification
- Various chromatography methods – affinity, HIC, dye-ligand
etc
Additional inoculum
- To give an idea of the amounts of virus inoculum required for protein
production, if a virus amplifies to 1 x 108 pfu/ml, we require 100 ml to
infect 1000 ml cells at an moi of 5 pfu/cell. If the titre is
only 5 x 107 pfu/ml, then 200 ml of virus will be required
NB Virus titres/Expression levels
OET cannot guarantee expression of any particular gene, nor can we guarantee
expression levels of proteins or virus titres. Virus titres are typically
in the range 4 x 107 to 1 x 108 pfu/ml. If the titre is exceptionally low
we will re-amplify and titrate at no extra cost.
|
