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NEXT DAY BACULOVIRUS TITRATION SERVICE

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Overview

Patent pending - No. 0607656.7

OET announce the release of baculoQUANT - a next-day baculovirus titration service. The new service was officially launched at the Baculovirus Technology Conference in Boston, U.S.A. on 25th September 2006.

“A fast, accurate and efficient means of obtaining viral titres is essential in the services we provide to pharmaceutical and biotechnology customers in the area of insect cell expression and in our ongoing development of the baculoworkstation. We have chosen to work with the quantitative Polymerase Chain Reaction (QPCR) viral titration service from Oxford Expression Technologies as this fulfils these criteria with knowledgeable, personable service.”
Dr. K S Richards, Senior Scientist, NextGen Sciences Ltd.

OET has developed a rapid, accurate and reproducible baculovirus titration method using quantitative real-time PCR (QPCR) specifically designed to the viral genome. The system has been calibrated against known viral titres, previously determined by plaque assay, and has been semi-automated using a Corbet Research liquid handler to prepare the QPCR reactions ready for quantification on an ABI 7500 Sequence Detection System.

References
  1. Hitchman RB, Siaterli EA, Nixon CP, King LA. 2006. Quantitative real-time PCR for rapid and accurate titration of recombinant baculovirus particles. Biotechnology and Bioengineering.
  2. Holland PM, Abramson RD, Watson R, Gelfand DH. 1991. Detection of specific polymerase chain reaction product by utilizing the 5'?3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. U.S.A. 88: 7276?7280.
  3. Heid CA, Stevens J, Livak KJ, Williams PM. 1996. Real time quantitative PCR. Genome Res. 6: 986?994.
Service Includes
  • Viral DNA extraction
  • QPCR preparation and quantification
  • Viral titration determination (using an algorithm previously determined by plaque assay)
  • Email of titres (Excel format)
Advantages of the baculoQUANT service
  • Avoids unnecessary wastage of viral stocks
  • Allows optimisation of viral amplification and protein production
  • Provides rapid and accurate titration of multiple viruses in 96-well format
  • Fast and simple to use
To order
  • email/phone to check availability
  • send 200ul FRESH baculovirus stock (must be less than 3 months old or reading will be inaccurate)
  • send/fax official purchase order
  • email/fax notice of despatch of virus
  • NB stock received by 12.00 midday will have results the following working day
  • Click here to view baculoQUANT Kits