All-in-one baculovirus expression and titration
A unique dual expression and titration package that harnesses the speed and power
of flashBACTM expression with the ease and rapidity of baculoQUANTTM titration
flashBACTM allows the generation of single and multiple recombinant viruses
in one easy step by homologous recombination in insect cells, without the need
for plaque purification (see flashBACTM Technical Description). baculoQUANTTM is
a rapid method for the titration of baculoviruses, being designed to give accurate
titres within 2 - 6 hours (depending on number of viruses)1,2.
titrePLUSTM is a new combination kit, bringing together both of
the above technologies into a single format, providing a unique ‘off-the-shelf’ solution
to making recombinant viruses.
Using this kit researchers can rapidly make single or multiple recombinant viruses
in a single step procedure and then obtain titre(s) on the same day as
harvest.
- Rapid production and titration of single and multiple recombinant
viruses in a single kit format
- Cost-efficient and economic with easy to follow
protocols
- flashBACTM is compatible with all transfer vectors based on
homologous recombination in insect cells
- Contains flashBACTM DNA, control
transfer vector, baculoQUANTTM probe/primers
- Available in 5 reaction
and 24 reaction size kits
Both flashBACTM and baculoQUANTTM are also available in separate
kit formats.
- Hitchman RB, Siaterli EA, Nixon CP, & King.
LA. Quantitative real-time PCR for rapid and accurate titration of recombinant
baculovirus particles. Biotechnol Bioeng. 2007 Mar 1;96(4):810-4.
- Hitchman
RB, Siaterli EA, Nixon CP, & King. LA.
A baculovirus titration system for high-throughput applications. Biotechnol Bioeng.
2007 Mar 1;96(4):Fmv.
For more information on commercial licences, please contact Helen Irving at
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