EXPERTS IN INSECT, MAMMALIAN AND BACTERIAL EXPRESSION

Co-transfection → Recombinant virus; services from OET

Are you looking for scientific solutions that help to ensure success when it comes to baculovirus co-transfections? Oxford Expression Technologies Ltd (OET) is a name you can trust to provide technical and realistic results. We are based in the heart of Oxford and provide services both in the UK and overseas. Trusted experts within the field of baculovirus co-transfection and home to the flashBAC™ baculovirus expression kit. Find out more below or get in touch with us for more information.

Covering every step of successful co-transfection.

Construction of the recombinant baculovirus via homologous recombination relies on the success of the co-transfection. OET offers a cost-effective approach to customers who wish to ensure success with their baculovirus co-transfection(s). For the co-transfection reaction, a transfer plasmid containing the gene of interest needs to be either provided by the customer or produced by OET. This transfer plasmid is then used with the one-step flashBAC™ baculoviral DNA to co-transfect insect cells. A control co-transfection is also performed in which a transfer plasmid containing either the lacZ or eGFP gene is used. Following incubation of cells for upto five days at 28°C, the cell culture medium containing the recombinant virus is harvested.

Optimised: Specific genomes available for difficult proteins.

Stable: High expression levels maintained across multiple passages.

GMP-ready: flashBAC™ Gold, Prime and Ultra2 viruses contain no bacterial sequences.

VLPs: flashBAC™ Prime delivers cleaner, higher yields for intracellular VLPs; Ultra for secreted VLPs

Expert Support: We’ll help you select the best flashBAC™ for your project.

The flashBAC™ System

flashBAC™ is a one-step baculovirus based protein expression platform that enables fast and simultaneous production of recombinant viruses suitable for the synthesis of both simple and complex proteins. Genetic optimization of the Autographa californica nucleopolyhedrovirus (AcMNPV) genome yields recombinant virus in a single step.