EXPERTS IN INSECT, MAMMALIAN AND BACTERIAL EXPRESSION
Mammalian services from OET
Here at Oxford Expression Technologies LTD (OET), the mammalian expression projects are performed using plasmid constructs that can be used both for transient and stable expression in several different mammalian cell lines. OET has access to a wide range of mammalian promoters to customise the level of gene expression in mammalian cells. Find out more below or get in touch now.
Contract services for baculovirus projects including mammalian expression
Like all Oxford Expression Technologies Ltd contract services, the baculovirus projects can cover every step from gene synthesis to protein optimisation, scale up and purification.
Gene synthesis and gene cloning
We can arrange the synthesis of any gene for you, and we’ll also work with any plasmid you supply as a starting point.
The plasmid containing the gene of interest is transfected into the target mammalian cell line and expression levels are determined by western blot.
Stable cell line
Stable cell line is produced following transfection of mammalian cells with the plasmid containing the gene of interest and a selectable antibiotic marker. Positive selection is used by the presences of the antibiotic to isolate and amplify recombinant mammalian cells, which contain the gene of interest.
Expression of recombinant protein
Following transient expression or generation of the stable cell line experiment, the protein produced can be purified or shipped direct as crude product.
OET can scale up the production, all the way to 20L using a WAVE machine bioreactor.
For further details on our mammalian services or a free quote, please get in touch with OET now.
BacMam protein expression services
Oxford Expression Technologies also offers BacMam expression service.
BacMam viruses can be constructed by OET using the flashBAC™ expression system. This service includes complete optimisation of expression for your protein of interest. BacMam protein expression services offer high quality proteins at a competitive price.
What are BacMam viruses?
BacMams are modified baculoviruses in which a mammalian promoter is used to drive gene expression in mammalian cells following baculoviral transduction. The system can be used to transduce a wide range of mammalian cell lines including HEK293, HeLa, U2-OS, COS and CHO cells. Transduction of mammalian cells with BacMam viruses has been shown to be highly efficient and dose dependent. Moreover, the ability of baculoviruses to enter mammalian cells means that the transduction can be achieved by simply adding a virus inoculum to the cells.
What are the advantages of the BacMam expression system?
The BacMam system offers several advantages over plasmid-based transfection and other virus-based gene expression systems. The key advantages include:
High level of biosafety as baculoviruses do not replicate in mammalian cells
Reduced overt cytotoxicity even at high virus concentration
High transduction efficiency maximises gene delivery into cells
Wide host range
A broad range of mammalian cell types can be transduced
Expression is much faster than generation of stable cell lines