EXPERTS IN INSECT, MAMMALIAN AND BACTERIAL EXPRESSION
The home of flashBAC™ technology. Buy online or contact us for more information
Welcome to Oxford Expression Technologies Ltd, where you can buy our flashBAC™ products. Using the flashBAC™ system is easier and quicker than other baculovirus expression systems because there is no need to separate recombinant virus from parental virus by plaque-purification or any other means; only recombinant virus is produced after the co-transfection. Because the production of recombinant virus has been reduced to a single step procedure in insect cells, it is amenable to high throughput and automated production systems. However, it is also of benefit to the small research group requiring a low cost solution to producing one or a few recombinant baculoviruses prepared in individual dishes of cells.
Ordering is easy and can be done using a credit card online or by submitting a purchase order number. Whether you want to buy now or even let us take the load off you and do the work in-house, all you need to do is get in touch!
The flashBAC™ System
flashBAC™ is a one-step baculovirus based protein expression platform that enables fast and simultaneous production of recombinant viruses suitable for the synthesis of both simple and complex proteins. Genetic optimization of the Autographa californica nucleopolyhedrovirus (AcMNPV) genome yields recombinant virus in a single step.
flashBAC™ is a streamlined process for the production of recombinant baculoviruses which is quicker and simpler then rival systems
The original flashBAC™ vector is a good choice for projects where the gene to be expressed is likely to be simple and targeted to the cytoplasm or nucleus of infected cells. The deletion of the chiA (chitinase) improves membrane and secreted protein production.
flashBAC™ GOLD provides superior levels of expression for any protein that is destined for secretion or to be inserted in the membrane, or for proteins that might be particularly liable to degradation. the deletion of chiA (chitinase) and v-cath (cathepsin) increases efficacy of the secreted pathway and reduces recombinant protein degredation.
In addition to chiA (chitinase) and v-cath (cathepsin), three more virus genes (p10, p74 and p26) have been excised from the flashBAC™ ULTRA genome. These deletions ensure a longer time frame for protein expression and creates a more efficient baculovirus expression vector.
flashBAC™ PRIME is based on the original AcMNPV genome without any of the gene deletions characteristic of the other flashBAC™ range of vectors. Thus cells infected with flashBAC™ PRIME induce cell lysis in the late stages of infection which facilitates release and subsequent purification of VLPs (or other proteins) that form in the cytoplasm or nucleus of infected cells.
flashBAC™ and Starter Kits
OET provides a range of baculovirus expression kits based on flashBAC, GOLD, ULTRA and PRIME. We also provide kits based on linear DNA technology - BacPAK6.