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Sf9 Insect Cells

Sf9 Insect Cells


Sf9 insect cells have become the workhorse of the baculovirus expression system, being used worldwide for the production of recombinant viruses and expression proteins.


Sf9 cells are a clonal isolate from Spodoptera frugiperda (Fall armyworm) IPLB-Sf21-AE cells. The Sf9 cells are adapted to serum free suspension culture in ESF 921™ media but are capable of conforming to other suitable media types. The cells can be used for transient or stable expression of recombinant proteins; as monolayers for transfection and production of recombinant baculovirus; or for the propagation of baculovirus stocks. Sf9 insect cells can easily be cultured as monolayer cultures in T-flasks, suspension cultures in shake flasks, or scaled up for use in bioreactors.

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