EXPERTS IN INSECT, MAMMALIAN AND BACTERIAL EXPRESSION
Transfection and Virus Titration
OET provides a non-toxic insect transfection reagent optimised for use in insect cells to enable successful transfection and production of high titres of recombinant viruses for subsequent amplification. Although optimised for insect cell lines such as Sf9, Sf21, T.ni and Drosophila S2 cells, the reagent will also work with other cell lines, including primary cells and various mammalian cell lines .
Due to the importance of generating an accurate virus titre for optimal recombinant protein production, we have developed a quick and accurate method for titration of your baculovirus stocks, using the rapid quantitative PCR (qPCR) product; baculoQUANT™ ALL-IN-ONE baculovirus titration kit,
BaculoCOMPLETE protein expression kit + Titration kit
It is essential that an accurate titre of virus be obtained to determine correct multiplicity of infection and to ensure optimal recombinant protein production. Traditionally, baculoviruses have been titrated by plaque assays or antibody based assays that requires 3-4 days. OET has teamed up with Agilent Technologies to introduce a revolutionary qPCR-based, baculovirus titration kit - baculoQUANT™ ALL-IN-ONE. By utilising qPCR, it is possible to accurately establish viral titres from large numbers of budded virus stocks in a number of hours. The kit includes all the components required to extract and quantify viral DNA. We also offer baculoQUANT™ ALL-IN-ONE as part of a package with baculoCOMPLETE so that you can conveniently carry out recombinant virus production and titration in one go.
BaculoFECTIN II and Transfection Medium
BaculoFECTIN II is a novel nanoparticle-based transfection reagent specifically developed and optimised for the transfection of insect cells. Non-toxic, simple to use, and efficient, baculoFECTIN II transfection reagent is an excellent choice for producing high titre recombinant virus stocks whilst maintaining cell health. Our transfection reagent is compatible with multiple cell lines and baculovirus expression vectors includuing our own flashBAC™ and BacPAK6 technology.