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pOET1.1C 6xHis Transfer Plasmid

pOET1.1C 6xHis Transfer Plasmid


Product code: 2001012

Company undertaking Identification:

Oxford Expression Technologies Ltd, BioInnovation Hub, Gipsy Lane Campus, Oxford OX3 0BP, UK

pOET1.1C 6xHis (cat# 2001012) (10ug) is a baculovirus transfer vector designed for high level expression of foreign genes under the powerful AcMNPV polyhedron (polh) promoter.  The vector encodes an optional C-terminal 6xHis-Tag® fusion sequence that may be utilised.  This greatly eases the purification of the recombinant protein since the 6xHis-containing fusion proteins bind with high affinity to Ni-NTA Agarose.  pOET1.1C has a ColE1 origin of replication and an ampicillin resistance gene for selection in E. coli. The polh sequences have been replaced by a multiple cloning site (MCS) containing unique restriction sites for insertion of the foreign gene in the correct orientation.  The AcMNPV sequences flanking the gene in the transfer vector’s MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus. pOET1.1C is compatible with any baculovirus system that utilises homologous recombination in insect cells.

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