Sequence errors in your gene for expression Part II follows on from an earlier blog where we discussed a problem we were having producing...

If you don’t achieve the levels of protein expression expected in your project, have you ever considered that there may be sequence...

Transferring Sf9 cells from monolayers to suspension cultures can sometimes be difficult.  These insect cells, commonly employed for...

One of the unexpected offshoots of the baculovirus expression system has been its development as a vehicle for the delivery of genes to...

For a follow up to our last blog we want to talk about maintaining healthy cells over the holiday period spanning Christmas and New Year....

Following on from our last blog on the revival of cells stored in liquid nitrogen, we now provide some tips on the continued healthy...

You may have had the experience!  An expression project is going really well.  You inserted your gene into a baculovirus vector...

We get asked to do many service projects for customers where they want to produce a modified form of a protein for structural/functional...

We told you last time about our trip to the Society for Invertebrate Pathology meeting in Tours, France.  This was another highly...

Ever wondered where the baculovirus insect cell expression system came from? The clue is in the name – of course it came from viruses...

Have you ever wondered why the baculovirus we use for most protein expression purposes is called AcMNPV?  This is short for Autographa...

Using baculovirus expression vectors to produce virus-like particles (VLPs) is not new.  Intact, non-infectious poliovirus1 and...

We had promised to consider the production of virus like particles in this blog, but have postponed this topic to a later date after the...

After scaling up recombinant baculovirus-insect cell cultures (blog) we now come to what can be a very tricky part of the operation,...

Once you have decided on the optimal conditions for producing your recombinant protein target in baculovirus-infected insect cells, your...

Once you have finished making your recombinant baculoviruses expression vector you naturally want to maximize the amount of protein made...

A question that comes up repeatedly is the long term stability of recombinant budded baculovirus stocks.  This is a particular issue now...

Knowing the infectious titre of a recombinant virus stock is really important prior to testing for protein production in insect cells. ...

We were going to devote this blog to a comprehensive account of how you might determine the infectious titre of your virus stock. ...

In our last blog post we talked about the process of transfecting insect cells with virus DNA and how you could recognise success or...