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EXPERTS IN BACULOVIRUS PROTEIN EXPRESSION

BAC TO THE FUTURE
Co-infection with Baculovirus Vectors for Multiple Protein Expression
A question that comes up from time to time is the use of co-infection with baculovirus vectors for multiple protein expression in insect...
yelluk
Oct 4, 20172 min read


Baculovirus Transfer Vectors Compatible with flashBAC™
A question we are asked from time to time is to identify baculovirus transfer vectors compatible with our flashBAC™ system. We obviously...
yelluk
Sep 20, 20172 min read
Variable Expression of Multi-Component Protein Complexes
Variable expression of multi-component protein complexes is increasingly necessary as more sophisticated projects are attempted. But how...
yelluk
Sep 8, 20172 min read


Intermediate scale recombinant protein production
Between initial tests and larger scale up you may want to conduct intermediate scale recombinant protein production using baculovirus...
yelluk
Jul 25, 20171 min read


Welcome!
Oxford Expression Technologies are the experts when it comes to the science of baculovirus. For almost 10 years now we have specialised...
yelluk
Jun 22, 20171 min read
Adapting Insect Cells to a New Growth Medium?
Have you ever had the experience of adapting insect cells to a new growth medium? We recently had to do this for a client project...
yelluk
Jun 21, 20172 min read
Baculovirus Expressed Secreted Recombinant Proteins
A question that often comes up from clients is what is the best signal peptide to use for baculovirus-expressed secreted recombinant...
yelluk
May 30, 20172 min read
baculoQUANT Titration of Recombinant Baculoviruses
There are many methods available for the titration of recombinant baculoviruses. One of the most convenient is the use of the...
yelluk
May 2, 20173 min read
Sequence Errors in Your Gene for Expression Part II?
Sequence errors in your gene for expression Part II follows on from an earlier blog where we discussed a problem we were having producing...
yelluk
Apr 26, 20172 min read
Sequence Errors in Your Gene for Expression?
If you don’t achieve the levels of protein expression expected in your project, have you ever considered that there may be sequence...
yelluk
Apr 3, 20173 min read


Sf9 Cells from Monolayers to Suspension Cultures
Transferring Sf9 cells from monolayers to suspension cultures can sometimes be difficult. These insect cells, commonly employed for...
yelluk
Feb 1, 20172 min read
Transduction of Whole Porcine Kidneys with BacMams
One of the unexpected offshoots of the baculovirus expression system has been its development as a vehicle for the delivery of genes to...
yelluk
Jan 26, 20172 min read
Healthy Cells Over the Holiday
For a follow up to our last blog we want to talk about maintaining healthy cells over the holiday period spanning Christmas and New Year....
yelluk
Nov 30, 20162 min read
Healthy Culture of Insect Cells
Following on from our last blog on the revival of cells stored in liquid nitrogen, we now provide some tips on the continued healthy...
yelluk
Nov 22, 20164 min read
Insoluble Proteins – Inconsolable!
You may have had the experience! An expression project is going really well. You inserted your gene into a baculovirus vector...
yelluk
Oct 14, 20162 min read
Protein Expression and Directed Mutations
We get asked to do many service projects for customers where they want to produce a modified form of a protein for structural/functional...
yelluk
Sep 22, 20163 min read
Louis Pasteur and Insect Viruses
We told you last time about our trip to the Society for Invertebrate Pathology meeting in Tours, France. This was another highly...
yelluk
Aug 18, 20163 min read
Bac to Our Roots!
Ever wondered where the baculovirus insect cell expression system came from? The clue is in the name – of course it came from viruses...
yelluk
Jul 22, 20162 min read
Baculovirus Gene Mutations and Protein Expression
Have you ever wondered why the baculovirus we use for most protein expression purposes is called AcMNPV? This is short for Autographa...
yelluk
Jul 14, 20163 min read
Producing Virus-Like Particles (VLPs) in Insect Cells
Using baculovirus expression vectors to produce virus-like particles (VLPs) is not new. Intact, non-infectious poliovirus1 and...
yelluk
Jun 29, 20163 min read
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