yelluk
Introduction to the Baculovirus Expression System
Baculoviruses are in many respects an odd choice for use as an expression system to make recombinant proteins. In their natural...
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EXPERTS IN BACULOVIRUS PROTEIN EXPRESSION
BAC TO THE FUTURE
yelluk
- Jun 11, 2019
- 2 min
Baculovirus P10 Structures and Role in Insect Cells
A blog on baculovirus P10 structures and the role in insect cells might appear a slightly odd topic for a blog from a company based on...
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yelluk
- Jul 9, 2018
- 2 min
Baculovirus Transfer Vector Plasmid Compatibility
A frequent customer query concerns baculovirus transfer vector plasmid compatibility with either flashBACTM or other systems for making...
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yelluk
- Sep 20, 2017
- 2 min
Baculovirus Transfer Vectors Compatible with flashBAC™
A question we are asked from time to time is to identify baculovirus transfer vectors compatible with our flashBAC™ system. We obviously...
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yelluk
- May 2, 2017
- 3 min
baculoQUANT Titration of Recombinant Baculoviruses
There are many methods available for the titration of recombinant baculoviruses. One of the most convenient is the use of the...
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yelluk
- Jan 26, 2017
- 2 min
Transduction of Whole Porcine Kidneys with BacMams
One of the unexpected offshoots of the baculovirus expression system has been its development as a vehicle for the delivery of genes to...
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yelluk
- Jul 22, 2016
- 2 min
Bac to Our Roots!
Ever wondered where the baculovirus insect cell expression system came from? The clue is in the name – of course it came from viruses...
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yelluk
- Jul 14, 2016
- 3 min
Baculovirus Gene Mutations and Protein Expression
Have you ever wondered why the baculovirus we use for most protein expression purposes is called AcMNPV? This is short for Autographa...
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yelluk
- Jun 29, 2016
- 3 min
Producing Virus-Like Particles (VLPs) in Insect Cells
Using baculovirus expression vectors to produce virus-like particles (VLPs) is not new. Intact, non-infectious poliovirus1 and...
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yelluk
- Apr 11, 2016
- 3 min
Longevity of Infectious Budded Baculovirus Stocks
A question that comes up repeatedly is the long term stability of recombinant budded baculovirus stocks. This is a particular issue now...
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yelluk
- Mar 31, 2016
- 3 min
Rapid Virus Titration by QPCR
Knowing the infectious titre of a recombinant virus stock is really important prior to testing for protein production in insect cells. ...
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yelluk
- Mar 11, 2016
- 4 min
Titrating Stocks of Recombinant Baculoviruses Using the Plaque Assay Technique
We were going to devote this blog to a comprehensive account of how you might determine the infectious titre of your virus stock. ...
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yelluk
- Feb 25, 2016
- 5 min
Production of High Titre Virus Stocks
In our last blog post we talked about the process of transfecting insect cells with virus DNA and how you could recognise success or...
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yelluk
- Feb 10, 2016
- 3 min
Transfection of Insect Cells with Baculovirus DNA
Independent of the method you use to produce recombinant viruses, at some point you have to transfect insect cells with virus DNA to...
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yelluk
- Jan 13, 2016
- 2 min
To heat treat or not to heat treat calf serum, that is the question?
Ok, so the Bard is probably spinning in his grave at our blatant plagiarism of the script from the “Scottish Play” (it is bad luck to use...
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yelluk
- Nov 13, 2015
- 2 min
Baculovirus Protein Expression Made Easy – flashBAC!
The flashBAC™ system is a revolutionary platform technology for the production of recombinant baculoviruses in insect cells. It comprises...
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yelluk
- Sep 23, 2014
- 2 min
Producing Your Recombinant Baculovirus
There are 2 main methods through which you can happily produce recombinant baculoviruses, firstly by using systems based on homologous...
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yelluk
- Aug 4, 2014
- 2 min
Baculovirus with Benefits: Expression System Advantages
As with all protein expression systems the baculovirus has its advantages and set-backs, the trade-off for high levels of expression,...
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yelluk
- Jul 17, 2014
- 3 min
Growing Pains: The Life Cycle of the Baculovirus
Thankfully baculoviruses don’t go through a rebellious teenage phase. They do however have a well-defined 2 step life cycle. Here we will...
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yelluk
- Jul 15, 2014
- 1 min
First Things First: What Exactly Is A Baculovirus?
A quick introduction into baculoviruses and baculovirus expression vectors, brought to you by OET. Short answer: An insect virus which...
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